Choice of Efficient Molecular Biology Techniques for Bacterial Cell Surface Display

For in vitro assessment of proteins, cell surface display is a novel technique. Using flow cytometry and / or iterative selection procedures like biopanning, libraries of heterologous polypeptides can de displayed and monitored on the surface of bacterial and yeast cells. However, this is not possible without the choice of efficient strains, reagents etc. and some high-throughput molecular biology techniques. In this short technical note, we discuss the use and reliability of some highly efficient vectors, reagents and techniques that may enhance the expression of metal binding proteins on the surface of Gram-negative bacterial cells. The apt choices of these will facililate the surface display approach as a tool for fundamental and applied research in frontier areas like Bioremediation, Vaccinology and